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Investigation:

ND-07 (Zepp / Bustamante)

LBA Dataset ID:

ND07_15N_LEAVES_SOIL

Originator(s):

1. BUSTAMANTE, M.M.C.
      2. MARTINELLI, L.A.

Point(s) of Contact:

ORNL DAAC User Services Office Oak Ridge National Laboratory Oak Ridge, Tennessee 37 (ornldaac@ornl.gov)

Dataset Abstract:

This dataset includes 15N and total nitrogen concentrations for foliar samples and soil samples collected in from cerrado sites within the IGBE Reserve, Brasilia. Foliar samples were collected from 320 individuals representing 45 species. Soil samples were collected from 5 locations (2 in campo sujo, 2 in cerrado denso and 1 in cerrado) to 450 cm depth in the campo sujo and 800 cm depth elsewhere.

Beginning Date:

1999-12-01

Ending Date:

2000-09-30

Metadata Last Updated on:

2012-09-21

Data Status:

Archived

Access Constraints:

PUBLIC

Data Center URL:

http://daac.ornl.gov/

Distribution Contact(s):

ORNL DAAC User Services Office Oak Ridge National Laboratory Oak Ridge, Tennessee 37 (ornldaac@ornl.gov)

Access Instructions:

PUBLIC

Data Access:

IMPORTANT: The LBA-ECO Project website is no longer being supported. Links to external websites may be inactive. Final data products from the LBA project can be found at the ORNL DAAC. Please follow the fair use guidelines found in the dataset documentation when using or citing LBA data.
Datafile(s):

LBA-ECO ND-07 Carbon and Nitrogen in Cerrado Plants and Soils, Brasilia: 1999-2000 :  http://daac.ornl.gov/cgi-bin/dsviewer.pl?ds_id=1121

Documentation/Other Supporting Documents:

LBA-ECO ND-07 Carbon and Nitrogen in Cerrado Plants and Soils, Brasilia: 1999-2000 :  http://daac.ornl.gov/LBA/guides/ND07_15N_Leaves_Soil.html

Citation Information - Other Details:

Bustamante, M.C.C. and L.A. Martinelli. 2012. LBA-ECO ND-07 Carbon and Nitrogen in Cerrado Plants and Soils, Brasilia: 1999-2000. Data set. Available on-line [http://daac.ornl.gov] from Oak Ridge National Laboratory Distributed Active Archive Center, Oak Ridge, Tennessee, U.S.A. http://dx.doi.org/10.3334/ORNLDAAC/1121

Keywords - Theme:

Parameter Topic Term Source Sensor
CARBON BIOSPHERE SOILS LABORATORY CHN ANALYZER
CARBON BIOSPHERE VEGETATION LABORATORY CARBON ANALYZER
CARBON BIOSPHERE SOILS LABORATORY CHN ANALYZER
CARBON BIOSPHERE VEGETATION LABORATORY CARBON ANALYZER
ISOTOPES BIOSPHERE VEGETATION LABORATORY MASS SPECTROMETER
NITROGEN BIOSPHERE SOILS LABORATORY CHN ANALYZER
NITROGEN BIOSPHERE VEGETATION LABORATORY CHN ANALYZER
NITROGEN BIOSPHERE SOILS LABORATORY CHN ANALYZER
NITROGEN BIOSPHERE VEGETATION LABORATORY CHN ANALYZER

Uncontrolled Theme Keyword(s):  BRASILIA, BRAZIL, CARBON, ISOTOPE RATIOS, NITROGEN, SOILS, VEGETATION

Keywords - Place (with associated coordinates):

Region
(click to view profile)
Site
(click to view profile)
North South East West
Brasília Reserva Ecologica do Roncador IBGE -15.93280 -15.93280 -47.85060 -47.85060

Related Publication(s):

Bustamante, M.M.C., L.A. Martinelli, D.A. Silva, P.B. Camargo, C.A. Klink, T.F. Domingues, R.V. Santos. 2004. 15N Natural Abundance in Woody Plants and Soils of Central Brazilian Savannas. Ecological Applications 14: S200-S213.

Data Characteristics (Entity and Attribute Overview):

Data Characteristics:

The data are available in two comma-delimited ASCII files titled:

File #1: Foliar_15N_IGBE_cerrado.csv.

File #2: Soil_isotope_ratios_IGBE_cerrado.csv



The data are organized as follows





File #1

File name,Foliar_15N_IGBE_cerrado.csv,,,,,



Column,Column_heading,Units/format,Explanation,,,

----1,Species,,Scientific name in the format Genus species,,,

----2,Family,,Scientific family,,,

----3,Spp_code,,Unique numeric code for each species,,,

----4,Physiognomy,,Vegetation type within the general classification of cerrado: Cerrado sensu strictu (cerrado); cerrado denso and campo sujo,,,

----5,Life_form,,Plant life form: tree;shrub or grass,,,

----6,delta_15N,per mil,Ratio of 15Nto 14N in the foliar sample expressed relative to standard air,,,

----7,N,percent,Foliar nitrogen concentration reported in percent of total leaf weight,,,



Example data records:



Species,Family,Spp_code,Physiognomy,Life_form,delta_15N,N

Acosmium dasycarpum,Leguminosae,30,cerrado,tree,0.8,3.6

Acosmium dasycarpum,Leguminosae,30,cerrado,tree,0.1,4.6

Acosmium dasycarpum,Leguminosae,30,cerrado,tree,-0.1,2.2

Acosmium dasycarpum,Leguminosae,30,cerrado,tree,-0.1,4.4

Acosmium dasycarpum,Leguminosae,30,cerrado,tree,-0.4,1.3

Acosmium dasycarpum,Leguminosae,30,cerrado,tree,-1.6,1





File #2

File name,Soil_isotope_ratios_IGBE_cerrado.csv,,,,,,,,



Column,Column_heading,Units/format,Explanation,,,,,,

----1,Sample_no,,Sample number: each field sample has a unique number; analytical replicates have the same sample number,,,,,,

----2,Sample_type,,Sample material: in addition to the soil samples 3 litter samples were collected from the soil surface near each pit,,,,,,

----3,Sample_code,,Unique sample identifier for each field sample,,,,,,

----4,Physiognomy,,Vegetation type within the general classification of cerrado: Cerrado sensu strictu (cerrado); cerrado denso and campo sujo based on samples collected by Tomas and Dulce,,,,,,

----5,Soil_pit_ID,,Samples were collected from one soil pit in the cerrado and two pits in each of the other two vegetation types,,,,,,

----6,Pit_wall,,Samples were collected from two walls in each pit at each depth walls were 150 cm apart from one another,,,,,,

----7,Analytical_rep,,Randomly samples were replicated for lab analysis and are indicated with an A or B,,,,,,

----8,Sampling_depth,,Depth below the soil surface from which the sample was collected,,,,,,

----9,Wt_sample,mg,Weight in milligrams of the sub sample used in the analysis,,,,,,

---10,delta_13C,per mil,Ratio of 13 C to 12 C in the sample relative to an international standard expressed in per mil,,,,,,

---11,C,percent,Concentration of carbon in the sample expressed as percent by weight,,,,,,,,,,,

---12,delta_15N ,per mil,Ratio of 15 C to 14 N in the sample relative to an international standard expressed as per mil,,,,,,,,,,,

---13,N,percent,Concentration of nitrogen in the sample expressed as percent by weight,,,,,,,,,,,

---14,C_to_N,,Ratio of carbon to nitrogen in the sample on a weight basis,,,,,,,





Example data records:



Sample_no,Sample_type,Sample_code,Physiognomy,Soil_pit_ID,Pit_wall,Analytical_rep,Sampling_depth,Wt_sample,delta_13C,C,delta_15N,N,C_to_N

1,Litter,CS1-1,campo sujo,1,not applicable,A,not applicable,1.575,-26.7,44.37,-0.16,0.73,60.68

1,Litter,CS1-1,campo sujo,1,not applicable,B,not applicable,1.517,-26.76,44.77,0.18,0.76,58.75

2,Litter,CS1-2,campo sujo,1,not applicable,-9999,not applicable,1.515,-26.83,49.1,1.2,0.64,76.95

3,Litter,CS1-3,campo sujo,1,not applicable,-9999,not applicable,1.58,-26.94,41.33,0.61,0.8,51.69

4,Litter,CS2-1,campo sujo,2,not applicable,-9999,not applicable,1.544,-26.84,45.8,0.94,0.77,59.7

5,Litter,CS2-2,campo sujo,2,not applicable,-9999,not applicable,1.551,-26.9,45.25,1.14,0.81,55.95

... records intentionally omitted ...

16,Soil,CS1A-1,campo sujo,1,A,A,0-5cm,15.524,-19.37,4.59,4.02,0.23,19.96

16,Soil,CS1A-1,campo sujo,1,A,B,0-5cm,15.055,-19.61,4.2,4.02,0.23,18.02

17,Soil,CS1A-2,campo sujo,1,A,-9999,5-10cm,15.403,-18.49,3.49,5.34,0.19,18.28

18,Soil,CS1A-3,campo sujo,1,A,-9999,10-20cm,25.505,-17.4,2.63,7.76,0.15,18.04

19,Soil,CS1A-4,campo sujo,1,A,-9999,20-30cm,25.16,-18.05,2.2,9.26,0.12,18.35

20,Soil,CS1A-5,campo sujo,1,A,-9999,30-40cm,25.296,-17.77,1.89,9.87,0.1,17.99

Data Application and Derivation:

15N isotope ratios and the variability in delta values within and across sites may be used as a proxy for interpreting aspects of the N cycles.

Quality Assessment (Data Quality Attribute Accuracy Report):

Quality Assessment:

Stable isotope ratios of nitrogen were measured relative to international recognized standards. Internal working standards (Atropine and soil standard no. 502-308 from LECO Corporation) were included in every run, as regular laboratory procedure. The precision of measurements was plus or minus 0.1 percent and 0.35 per mil for N and delta 15N, respectively

Process Description:

Data Acquisition Materials and Methods:

Site description:



Plant and soil samples were collected in the Ecological Reserve of the Brazilian Institute of Geography and Statistic (IBGE). This reserve has an area of 1350 ha and is located in the Planalto Central Brasileiro, approximately 35 km south of the city of Brasilia. The IBGE Reserve encompasses the most common vegetation types of cerrado: campo sujo, cerrado sensu stricto (cerrado), and cerrado denso. Eiten (1972) described campo sujo as an open savanna with scattered trees and shrubs, cerrado sensu stricto as a savanna woodland with abundant evergreen and deciduous trees and shrubs and an herbaceous under- story and cerrado denso as medium to tall woodlands with closed or semiclosed canopies.



Soils in the cerrado and cerrado denso are classified as Latossolo Vermelho-Escuro in the Brazilian soil classification, which is equivalent to Plinthic Haplustox in the American soil classification. In the campo sujo, the soil type is Latossolo Vermelho-Amarelo (Typic Haplustox). Typically, the soils are deep and acidic, with low organic matter and nutrient contents. The average annual rainfall is -1500 mm, most of it (>90%) falling in the rainy season, which results in severe water stress during the dry season (May to September).





Field methods:

We collected leaf samples of 320 individuals belonging to 45 species of woody plants including trees and shrubs. In the most intensive sampling site (cerrado) we collected 188 individuals of 45 species. In the cerrado denso and campo sujo, we collected 67 and 64 individuals, respectively, belonging to 32 species. We collected five fully expanded leaves from each plant. All samples from the cerrado denso and campo sujo and some of the samples in the cerrado were collected during the rainy season (December 1999 to March 2000). Additional samples for seasonal comparisons were collected in the cerrado during the dry season (July to September 2000). Leaves were air dried and ground for isotopic analysis.



We collected soil samples from two soil pits located in the campo sujo and in the cerrado denso, and from one soil pit located in the cerrado. At each depth interval, samples from two walls were taken; the pit walls are 150 cm apart. In the campo sujo, we sampled from surface to 50 cm deep at 10-cm intervals, from 50 cm to 150 cm at 50-cm intervals, and from 150 cm to 450 cm at 100-cm intervals. In the cerrado and cerrado denso, the same scheme was followed but we sampled to a depth of 800 cm.



Lab methods:

All foliar samples were oven-dried at 65 degrees C until constant weight and then ground to a fine powder. Soil samples were air-dried, sieved using a 2-mm mesh and homogenized. A smaller sub-sample was taken, handpicked to remove fine roots and other debris and then pounded. A 1-2 mg sub-sample of ground material or 15-20 mg sub-sample of pounded soil were placed and sealed in a tin capsule and loaded into a ThermoQuest-Finnigan Delta Plus isotope ratio mass spectrometer (Finnigan-MAT; CA, USA) in line with an Elemental Analyzer (Carla Erba model 1110; Milan, Italy) at the Laboratory of Isotope Ecology (CENA-USP, Brazil). From these analyzes we obtained the delta 15N ratio and tissue nitrogen content.

References:

Eiten, G. 1972. The Cerrado vegetation of Brazil. Botanical Review 38:201-341.

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